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anillin gfp rhoa biosensor  (Addgene inc)


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    Addgene inc anillin gfp rhoa biosensor
    Fig. 6 | CD97 promotes cell membrane retraction through activation of <t>RhoA.</t> a, HEK293T cells were transfected with CD97 (WT)–GFP, CD97 (T419G)–GFP or CTF–GFP fusion constructs. Longest diameters of individual WT- (n = 62), T419G- (n = 62) and CTF-transfected (n = 63) cells are shown. Each symbol represents one cell. One of three independent experiments with similar results is shown. b, Schematic diagram of an optically trapped ligand-coated bead applying pulling force while in contact with the cell membrane. The cell is also indicated to contain an anillin–GFP RhoA biosensor. c,d, HEK293T cells were cotransduced with anillin–GFP RhoA biosensor and CD97 (WT)–Scarlet, CD97 (T419G)–Scarlet or CD97 (PBM)–Scarlet fusion lentivirus and sorted. c, Cell trap forces of individual WT- (n = 70), T419G- (n = 64) and PBM-transduced (n = 62) cells. d, Normalized active RhoA biosensor fluorescence intensity of WT- (n = 27),
    Anillin Gfp Rhoa Biosensor, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anillin+gfp+rhoa+biosensor/pEGFP-RhoA+Biosensor+(Plasmid+%2368026)/pm38049580-490-0-16
    Average 93 stars, based on 22 article reviews
    anillin gfp rhoa biosensor - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Dynamic encounters with red blood cells trigger splenic marginal zone B cell retention and function."

    Article Title: Dynamic encounters with red blood cells trigger splenic marginal zone B cell retention and function.

    Journal: Nature immunology

    doi: 10.1038/s41590-023-01690-z

    Fig. 6 | CD97 promotes cell membrane retraction through activation of RhoA. a, HEK293T cells were transfected with CD97 (WT)–GFP, CD97 (T419G)–GFP or CTF–GFP fusion constructs. Longest diameters of individual WT- (n = 62), T419G- (n = 62) and CTF-transfected (n = 63) cells are shown. Each symbol represents one cell. One of three independent experiments with similar results is shown. b, Schematic diagram of an optically trapped ligand-coated bead applying pulling force while in contact with the cell membrane. The cell is also indicated to contain an anillin–GFP RhoA biosensor. c,d, HEK293T cells were cotransduced with anillin–GFP RhoA biosensor and CD97 (WT)–Scarlet, CD97 (T419G)–Scarlet or CD97 (PBM)–Scarlet fusion lentivirus and sorted. c, Cell trap forces of individual WT- (n = 70), T419G- (n = 64) and PBM-transduced (n = 62) cells. d, Normalized active RhoA biosensor fluorescence intensity of WT- (n = 27),
    Figure Legend Snippet: Fig. 6 | CD97 promotes cell membrane retraction through activation of RhoA. a, HEK293T cells were transfected with CD97 (WT)–GFP, CD97 (T419G)–GFP or CTF–GFP fusion constructs. Longest diameters of individual WT- (n = 62), T419G- (n = 62) and CTF-transfected (n = 63) cells are shown. Each symbol represents one cell. One of three independent experiments with similar results is shown. b, Schematic diagram of an optically trapped ligand-coated bead applying pulling force while in contact with the cell membrane. The cell is also indicated to contain an anillin–GFP RhoA biosensor. c,d, HEK293T cells were cotransduced with anillin–GFP RhoA biosensor and CD97 (WT)–Scarlet, CD97 (T419G)–Scarlet or CD97 (PBM)–Scarlet fusion lentivirus and sorted. c, Cell trap forces of individual WT- (n = 70), T419G- (n = 64) and PBM-transduced (n = 62) cells. d, Normalized active RhoA biosensor fluorescence intensity of WT- (n = 27),

    Techniques Used: Membrane, Activation Assay, Transfection, Construct, Fluorescence

    Related Articles

    Clone Assay:

    Article Title: Dynamic encounters with red blood cells trigger splenic marginal zone B cell retention and function.
    Article Snippet: .. Anillin–GFP RhoA biosensor was cloned from pEGFP-RhoA Biosensor (a gift from M. Glotzer, University of Chicago, Addgene plasmid 68026) and inserted into the lentiviral vector. .. Lentivirus were generated by mixing the plasmids HIV-gag pol (4 μg), vesicular stomatitis virus G protein (0.57 μg) and the respective lentiviral constructs (4 μg) and incubating this transfection mix in optimum medium (Thermo Fisher) with 4 μl of Xtremegene (Clontech) for 20 min at room temperature.

    Article Title: Dynamic encounters with red blood cells trigger splenic marginal zone B cell retention and function
    Article Snippet: .. Anillin–GFP RhoA biosensor was cloned from pEGFP-RhoA Biosensor (a gift from M. Glotzer, University of Chicago, Addgene plasmid 68026) and inserted into the lentiviral vector. .. Lentivirus were generated by mixing the plasmids HIV-gag pol (4 μg), vesicular stomatitis virus G protein (0.57 μg) and the respective lentiviral constructs (4 μg) and incubating this transfection mix in optimum medium (Thermo Fisher) with 4 μl of Xtremegene (Clontech) for 20 min at room temperature.

    Plasmid Preparation:

    Article Title: Dynamic encounters with red blood cells trigger splenic marginal zone B cell retention and function.
    Article Snippet: .. Anillin–GFP RhoA biosensor was cloned from pEGFP-RhoA Biosensor (a gift from M. Glotzer, University of Chicago, Addgene plasmid 68026) and inserted into the lentiviral vector. .. Lentivirus were generated by mixing the plasmids HIV-gag pol (4 μg), vesicular stomatitis virus G protein (0.57 μg) and the respective lentiviral constructs (4 μg) and incubating this transfection mix in optimum medium (Thermo Fisher) with 4 μl of Xtremegene (Clontech) for 20 min at room temperature.

    Article Title: Dynamic encounters with red blood cells trigger splenic marginal zone B cell retention and function
    Article Snippet: .. Anillin–GFP RhoA biosensor was cloned from pEGFP-RhoA Biosensor (a gift from M. Glotzer, University of Chicago, Addgene plasmid 68026) and inserted into the lentiviral vector. .. Lentivirus were generated by mixing the plasmids HIV-gag pol (4 μg), vesicular stomatitis virus G protein (0.57 μg) and the respective lentiviral constructs (4 μg) and incubating this transfection mix in optimum medium (Thermo Fisher) with 4 μl of Xtremegene (Clontech) for 20 min at room temperature.



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    Fig. 6 | CD97 promotes cell membrane retraction through activation of <t>RhoA.</t> a, HEK293T cells were transfected with CD97 (WT)–GFP, CD97 (T419G)–GFP or CTF–GFP fusion constructs. Longest diameters of individual WT- (n = 62), T419G- (n = 62) and CTF-transfected (n = 63) cells are shown. Each symbol represents one cell. One of three independent experiments with similar results is shown. b, Schematic diagram of an optically trapped ligand-coated bead applying pulling force while in contact with the cell membrane. The cell is also indicated to contain an anillin–GFP RhoA biosensor. c,d, HEK293T cells were cotransduced with anillin–GFP RhoA biosensor and CD97 (WT)–Scarlet, CD97 (T419G)–Scarlet or CD97 (PBM)–Scarlet fusion lentivirus and sorted. c, Cell trap forces of individual WT- (n = 70), T419G- (n = 64) and PBM-transduced (n = 62) cells. d, Normalized active RhoA biosensor fluorescence intensity of WT- (n = 27),
    Anillin Gfp Rhoa Biosensor, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anillin+gfp+rhoa+biosensor/pEGFP-RhoA+Biosensor+(Plasmid+%2368026)/pm38049580-490-0-16
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    gfp anillin  (Addgene inc)
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    Fig. 6 | CD97 promotes cell membrane retraction through activation of <t>RhoA.</t> a, HEK293T cells were transfected with CD97 (WT)–GFP, CD97 (T419G)–GFP or CTF–GFP fusion constructs. Longest diameters of individual WT- (n = 62), T419G- (n = 62) and CTF-transfected (n = 63) cells are shown. Each symbol represents one cell. One of three independent experiments with similar results is shown. b, Schematic diagram of an optically trapped ligand-coated bead applying pulling force while in contact with the cell membrane. The cell is also indicated to contain an anillin–GFP RhoA biosensor. c,d, HEK293T cells were cotransduced with anillin–GFP RhoA biosensor and CD97 (WT)–Scarlet, CD97 (T419G)–Scarlet or CD97 (PBM)–Scarlet fusion lentivirus and sorted. c, Cell trap forces of individual WT- (n = 70), T419G- (n = 64) and PBM-transduced (n = 62) cells. d, Normalized active RhoA biosensor fluorescence intensity of WT- (n = 27),
    Gfp Anillin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Fig. 6 | CD97 promotes cell membrane retraction through activation of RhoA. a, HEK293T cells were transfected with CD97 (WT)–GFP, CD97 (T419G)–GFP or CTF–GFP fusion constructs. Longest diameters of individual WT- (n = 62), T419G- (n = 62) and CTF-transfected (n = 63) cells are shown. Each symbol represents one cell. One of three independent experiments with similar results is shown. b, Schematic diagram of an optically trapped ligand-coated bead applying pulling force while in contact with the cell membrane. The cell is also indicated to contain an anillin–GFP RhoA biosensor. c,d, HEK293T cells were cotransduced with anillin–GFP RhoA biosensor and CD97 (WT)–Scarlet, CD97 (T419G)–Scarlet or CD97 (PBM)–Scarlet fusion lentivirus and sorted. c, Cell trap forces of individual WT- (n = 70), T419G- (n = 64) and PBM-transduced (n = 62) cells. d, Normalized active RhoA biosensor fluorescence intensity of WT- (n = 27),

    Journal: Nature immunology

    Article Title: Dynamic encounters with red blood cells trigger splenic marginal zone B cell retention and function.

    doi: 10.1038/s41590-023-01690-z

    Figure Lengend Snippet: Fig. 6 | CD97 promotes cell membrane retraction through activation of RhoA. a, HEK293T cells were transfected with CD97 (WT)–GFP, CD97 (T419G)–GFP or CTF–GFP fusion constructs. Longest diameters of individual WT- (n = 62), T419G- (n = 62) and CTF-transfected (n = 63) cells are shown. Each symbol represents one cell. One of three independent experiments with similar results is shown. b, Schematic diagram of an optically trapped ligand-coated bead applying pulling force while in contact with the cell membrane. The cell is also indicated to contain an anillin–GFP RhoA biosensor. c,d, HEK293T cells were cotransduced with anillin–GFP RhoA biosensor and CD97 (WT)–Scarlet, CD97 (T419G)–Scarlet or CD97 (PBM)–Scarlet fusion lentivirus and sorted. c, Cell trap forces of individual WT- (n = 70), T419G- (n = 64) and PBM-transduced (n = 62) cells. d, Normalized active RhoA biosensor fluorescence intensity of WT- (n = 27),

    Article Snippet: Anillin–GFP RhoA biosensor was cloned from pEGFP-RhoA Biosensor (a gift from M. Glotzer, University of Chicago, Addgene plasmid 68026) and inserted into the lentiviral vector.

    Techniques: Membrane, Activation Assay, Transfection, Construct, Fluorescence